Therefore, before you begin your analysis, it is important to first find out as much as possible about the cells you are analyzing. Your gating strategy is informed by what you know about your cells of interest. Learn as much as possible about your cells of interest So what gating methods do you need to know to confidently analyze your stained samples? This blog post will take you through the various gating strategies for effective flow cytometry analysis. This process of gating can appear quite random to a flow cytometry novice but it is in fact the most important part of flow cytometry analysis. save the new workspace and test it a few times to make sure it’s got all the pertinent info migrated over.Flow cytometry analysis typically begins with creating gates to distinguish cells of interest.repeat step d with layouts – open the two layout windows side by side and drag the layouts over.Drag table definitions (the names of tables from the list, the default one might be something like “untitled-1”) from one document to the other. Do this until all of your gates and groups are in place as originally. Click on the FCS data on the new workspace, Edit-Paste the gates into place. select your gates or gate-trees in the broken workspaces and Edit/Copy them.put your FCS files in the brand new workspace. ![]() Notice the “broken” one still shows all the gates, layouts, tables, they just won’t refresh or paint data.
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